In situ visualization of endothelial cell-derived extracellular vesicle formation in steady state and malignant conditions.

ABSTRACT: Endothelial cells are integral components of all vasculature within complex organisms. As they line the blood vessel wall, endothelial cells are constantly exposed to a variety of molecular factors and shear force that can induce cellular damage and stress. However, how endothelial cells are removed or eliminate unwanted cellular contents, remains unclear. The generation of large extracellular vesicles (EVs) has emerged as a key mechanism for the removal of cellular waste from cells that are dying or stressed. Here, we used intravital microscopy of the bone marrow to directly measure the kinetics of EV formation from endothelial cells in vivo under homoeostatic and malignant conditions. These large EVs are mitochondria-rich, expose the 'eat me' signal phosphatidylserine, and can interact w

SUBMITTER: Atkin-Smith GK 

PROVIDER: S-EPMC11496675 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature