Optimization of a novel expression system for recombinant protein production in CHO cells.

ABSTRACT: Chinese hamster ovary (CHO) cells are common mammalian cell lines for expressing recombinant proteins, yet the expression level of recombinant proteins is still hindered. Vector optimization and cell line modification are the key factors to improve the expression of recombinant proteins. In this study, the vector was optimized by adding the regulatory elements Kozak and Leader to the upstream of target gene to detect the transient and stable expression of recombinant proteins. Results indicated that the expression level of target proteins with the addition of regulatory elements was significantly increased compared with the control group. In addition, the inhibition of apoptotic pathway has great potential to increase recombinant protein production, and Apaf1 protein dependent on the mitoc

SUBMITTER: Zhang J 

PROVIDER: S-EPMC11496728 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature