Single-cell sequencing reveals immune features of treatment response to neoadjuvant immunochemotherapy in esophageal squamous cell carcinoma.

ABSTRACT: Neoadjuvant immunochemotherapy (nICT) has dramatically changed the treatment landscape of operable esophageal squamous cell carcinoma (ESCC), but factors influencing tumor response to nICT are not well understood. Here, using single-cell RNA sequencing paired with T cell receptor sequencing, we profile tissues from ESCC patients accepting nICT treatment and characterize the tumor microenvironment context. CXCL13⁺CD8⁺ Tex cells, a subset of exhausted CD8⁺ T cells, are revealed to highly infiltrate in pre-treatment tumors and show prominent progenitor exhaustion phenotype in post-treatment samples from responders. We validate CXCL13⁺CD8⁺ Tex cells as a predictor of improved response to nICT and reveal CXCL13 to potentiate anti-PD-1 effic

SUBMITTER: Yang Z 

PROVIDER: S-EPMC11496748 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature