CRISPR/dCas13(Rx) Derived RNA N⁶-methyladenosine (m⁶A) Dynamic Modification in Plant.

ABSTRACT: N⁶-methyladenosine (m⁶A) is the most prevalent internal modification of mRNA and plays an important role in regulating plant growth. However, there is still a lack of effective tools to precisely modify m⁶A sites of individual transcripts in plants. Here, programmable m⁶A editing tools are developed by combining CRISPR/dCas13(Rx) with the methyltransferase GhMTA (Targeted RNA Methylation Editor, TME) or the demethyltransferase GhALKBH10 (Targeted RNA Demethylation Editor, TDE). These editors enable efficient deposition or removal of m⁶A modifications at targeted sites of endo-transcripts GhECA1 and GhDi19 within a broad editing window ranging from 0 to 46 nt. TDE editor significantly decreases m⁶A levels by 24%-76%, while th

SUBMITTER: Yu L 

PROVIDER: S-EPMC11497087 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature