A Rapid Virus-Free Method for Producing Influenza HA Immunogen Needed for Preparation of Influenza Vaccine Potency Antisera Reagents.

ABSTRACT:

Background

The potency of inactivated and recombinant influenza vaccines is measured using the single-radial immunodiffusion (SRID) assay. The strain-specific antigen and antibody potency reagents required for the assay are prepared and distributed by regulatory agencies to ensure vaccine standardization, but timely reagent production is always challenging. This poses unique concerns for rapid pandemic responses. Alternative methods have been described for generating strain-specific potency antibody reagents without the need for live influenza virus, but such methods are infrequently used, suggesting the need for additional antigen expression approaches.

Methods

We describe a rapid process using a mammalian expression system to produce recombinant influenza hemagglutinin (rH

SUBMITTER: Odin M 

PROVIDER: S-EPMC11497102 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature