Development of a luminescence-based method for measuring West Nile Virus MTase activity and its application to screen for antivirals.

ABSTRACT: West Nile virus (WNV) is a flavivirus responsible for causing febrile illness and severe neurological diseases, with an increasing impact on human health around the world. However, there is still no adequate therapeutic treatment available to struggle WNV infections. Therefore, there is an urgent need to develop new techniques to accelerate the discovery of drugs against this pathogen. The main protein implicated in the replication of WNV is the non-structural protein 5 (NS5). This multifunctional protein contains methyltransferase (MTase) activity involved in the capping formation at the 5'-end of RNA and the methylation of internal viral RNA residues, both functions being essential for viral processes, such as RNA translation and escape from the innate immune response. We have developed

SUBMITTER: Alvarez-Minguez A 

PROVIDER: S-EPMC11497361 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature