Viability discrimination of bacterial microbiomes in home kitchen dish sponges using propidium monoazide treatment.

ABSTRACT: Dish sponges are known to support the proliferation of human bacterial pathogens, yet they are commonly used by consumers. Exposure to foodborne pathogens via sponge use may lead to illness, a serious concern among susceptible populations. The extent of exposure risks from sponge use has been limited by constraints associated with culture-independent or dependent methods for bacterial community characterization. Therefore, five used dish sponges were characterized to evaluate the presence of viable bacterial foodborne pathogens using the novel application of propidium monoazide (PMA) treatment and targeted 16S rRNA gene amplicon sequencing. Select pathogen viability was confirmed using targeted selective enrichment. The taxonomic abundance profiles of total and viable sponge microbiomes di

SUBMITTER: Carstens CK 

PROVIDER: S-EPMC11497490 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature