MicroRNAs from Yishen Tongluo formula can repair sperm DNA damage caused by benzo(<i>a</i>)pyrene.

ABSTRACT:

Context

Plant microRNAs (miRNAs) present in Yishen Tongluo formula (YSTL, a traditional Chinese herbal medicine formula) are considered as potential therapeutic drugs for reducing the sperm DNA fragmentation index (DFI).

Objective

To study the effectiveness of plant miRNAs in YSTL for repairing mouse sperm DNA damage caused by benzo(a)pyrene (BaP).

Methods and materials

Twenty-four male SPF ICR (CD1) mice were divided into control, BaP and YSTL groups. A BaP-induced (100 mg/kg) sperm DNA damage model was established in the BaP and YSTL groups, and the mice in the YSTL group were treated with YSTL (23.78 g/kg) for 8 weeks. Sperm DFI was determined via a sperm chromatin structure assay (SCSA). MicroRNAs in the testes of the mice were analysed via RN

SUBMITTER: Zhang C 

PROVIDER: S-EPMC11497566 | biostudies-literature | 2024 Dec

REPOSITORIES: biostudies-literature