Real-time monitoring of small extracellular vesicles (sEVs) by in vivo flow cytometry.

ABSTRACT: Extracellular vesicles (EVs) are vesicular structures comprised of a bilayer lipid membrane, released by living cells. There is a growing body of evidence for their functionality, indicating that small EVs (sEVs) can mediate specific forms of intercellular communication. The future applications of sEVs hold great promise, not only as diagnostic markers but also as therapeutic agents. However, the greatest difficulty in the clinical translation of sEVs is that they are currently poorly understood, especially concerning their in vivo behaviour. In this study, we provide a novel method for monitoring sEVs in blood circulation based on in vivo flow cytometry (IVFC). We have demonstrated that the height of the IVFC signal baseline is proportional to the concentration of sEVs. Moreover, we have

SUBMITTER: Zhang F 

PROVIDER: S-EPMC11497658 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature