Separation of small extracellular vesicles (sEV) from human blood by Superose 6 size exclusion chromatography.

ABSTRACT: Extracellular vesicles (EVs) are valuable targets for liquid biopsy. However, attempts to introduce EV-based biomarkers into clinical practice have not been successful to the extent expected. One of the reasons for this failure is the lack of reliable methods for EV baseline purification from complex biofluids, such as cell-free plasma or serum. Because available one-step approaches for EV isolation are insufficient to purify EVs, the majority of studies on clinical samples were performed either on a mixture of EVs and lipoproteins, whilst the real number of EVs and their individual specific biomarker content remained elusive, or on a low number of samples of sufficient volume to allow elaborate 2-step EV separation by size and density, resulting in a high purity but utmost low recovery. H

SUBMITTER: Nouvel J 

PROVIDER: S-EPMC11497763 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature