Diversity of <i>Clostridioides difficile</i> PCR ribotypes isolated from freshwater sediments depends on the isolation method.

ABSTRACT: Clostridioides difficile is an intestinal pathogen of humans and animals. In community-associated infections, the environment is suggested to play a significant role in overall transmission routes. Although the prevalence of C. difficile in freshwater and soil has been widely studied, little is known about its presence in sediments. In this study, we tested 15 sediment samples collected from various freshwater sources. C. difficile was isolated from all sampled sites, yielding a total of 171 strains grouped into 26 ribotypes, with 001/072 and 014/020 being the most prevalent. Genome sequencing of 37 isolates from 17 PCR ribotypes confirmed the presence of highly related strains in the geographically distant and unlinked water samples. Eight divergent PCR ribotypes from

SUBMITTER: Rupnik M 

PROVIDER: S-EPMC11497773 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature