Construction and characterization of DNA libraries from cultured phages and environmental viromes.

ABSTRACT: Despite many efforts to understand and leverage the functional potential of environmental viromes, most bacteriophage genes are largely uncharacterized. To explore novel biology from uncultivated microbes like phages, metagenomics has emerged as a powerful tool to directly mine new genes without the need to culture the diverse microbiota and the viruses within. When a pure computational approach cannot infer gene function, it may be necessary to create a DNA library from environmental genomic DNA, followed by the screening of that library for a particular function. However, these screens are often initiated without a metagenomic analysis of the completed DNA library being reported. Here, we describe the construction and characterization of DNA libraries from a single cultured phage (ΦT4),

SUBMITTER: Gu Liu C 

PROVIDER: S-EPMC11497775 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature