Transgenic overexpression of bmo-miR-6498-5p increases resistance to <i>Nosema bombycis</i> in the silkworm, <i>Bombyx mori</i>.

ABSTRACT: Microsporidia are unfriendly microorganisms, and their infections cause considerable damage to economically or environmentally important insects like silkworms and honeybees. Thus, the identification of measures to improve host resistance to microsporidia infections is critically needed. Here, an overexpressed miR-6498-5p transgenic silkworm line was constructed. Importantly, the survival rates and median lethal doses of the transgenic line were clearly higher after infection with Nosema bombycis. H&E staining and RT-qPCR analyses revealed an inhibitory effect on the proliferation of N. bombycis in the transgenic larvae. Metabolomics analysis further revealed the presence of 56 differential metabolites between the two lines. KEGG analysis of these 56 metabolites found that th

SUBMITTER: Hu C 

PROVIDER: S-EPMC11497792 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature