Modified Tn<i>7</i> transposon vectors for controlled chromosomal gene expression.

ABSTRACT: Complementation remains a foundation for demonstrating molecular Koch's postulates. While this is frequently achieved using plasmids, limitations such as increased gene copy number and the need for antibiotic supplementation to avoid plasmid loss can restrict their use. Chromosomal integration systems using the Tn7 transposon provide an alternative to plasmids for complementation and facilitate the stable insertion of genes at the chromosomal attTn7 site without the need for selection pressure. Here, we enhanced the utility of mini-Tn7 insertion vectors by the addition of inducible (Pcym) and constitutive (PcL and PrpsM) promoters, allowing differential transcriptional control of genes integrated into the chromosome. We validated the utility of the

SUBMITTER: Chang C 

PROVIDER: S-EPMC11497813 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature