Hyaluronate Protects From Benzalkonium Chloride-Induced Ocular Surface Toxicity.

ABSTRACT:

Purpose

The purpose of this study was to investigate the effect of sodium hyaluronate (SH) on benzalkonium chloride (BAK)-induced toxicity in the ocular surface epithelium and corneal nerves.

Methods

Ocular surface epithelial cells from Balb/c mice were cultured with 0.1% to 0.4% SH and 0.001% to 0.01% BAK and their metabolic activity, viability, and wound repair capacity were assessed in vitro. Following a controlled corneal wound, re-epithelialization and recovery of epithelial barrier function and mechanosensitivity were measured in Balb/c mice treated with 0.4% SH 3 times/day and 0.01% BAK twice daily for 3 weeks. Nerve morphology was assessed by confocal microscopy of corneal whole mounts.

Results

Whereas BAK exposure reduced metabolic activity, viability, and wo

SUBMITTER: Vereertbrugghen A 

PROVIDER: S-EPMC11498636 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature