Molecular communication between red blood cells of different phenotypes during blood storage in mice.

ABSTRACT:

Background

The cellular and molecular changes during red blood cell (RBC) storage that affect posttransfusion recovery (PTR) remain incompletely understood. We have previously reported that RBCs of different storage biology cross-regulate each other when stored together (co-storage cross-regulation [CSCR]). However, the mechanism of CSCR is unclear. In the current study, we tested the hypothesis that CSCR involves acquisition of molecular signatures associated with PTR.

Study design and methods

The whole blood compartment of either B6 or FVB mice was biotinylated in vivo prior to blood collection and storage. Bio-B6 or Bio.FVB were stored with RBCs from B6 mice transgenic for green florescent protein (GFP) (B6.GFP). After storage, avidin-magnetic beads were used to simultane

SUBMITTER: Zimring JC 

PROVIDER: S-EPMC11499017 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature