Parallel molecular data storage by printing epigenetic bits on DNA.

ABSTRACT: DNA storage has shown potential to transcend current silicon-based data storage technologies in storage density, longevity and energy consumption^1-3. However, writing large-scale data directly into DNA sequences by de novo synthesis remains uneconomical in time and cost⁴. We present an alternative, parallel strategy that enables the writing of arbitrary data on DNA using premade nucleic acids. Through self-assembly guided enzymatic methylation, epigenetic modifications, as information bits, can be introduced precisely onto universal DNA templates to enact molecular movable-type printing. By programming with a finite set of 700 DNA movable types and five templates, we achieved the synthesis-free writing of approximately 275,000 bits on an automated platform with 350 bi

SUBMITTER: Zhang C 

PROVIDER: S-EPMC11499255 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature