3D cultivation of non-small-cell lung cancer cell lines using four different methods.

ABSTRACT:

Purpose

The aim of this study was to set up reliable and reproducible culture conditions for 3D tumoroids derived from non-small cell lung cancer (NSCLC) cell lines to enable greater opportunity for successful cultivation of patient-derived samples.

Methods

Four NSCLC cell lines, two adenocarcinomas (A549, NCI-H1975) and two squamous cell carcinomas (HCC-95, HCC-1588), were first cultured in traditional 2D settings. Their expected expression profiles concerning TTF-1, CK7, CK5, and p40 status were confirmed by immunohistochemistry (IHC) before the generation of 3D cultures. Tumoroids were established in the hydrogel GrowDex^®-T, Nunclon™ Sphera™ flasks, BIOFLOAT™ plates, and Corning^® Elplasia^® plates. Western blot was used to verify antigen pr

SUBMITTER: Malmros K 

PROVIDER: S-EPMC11499447 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature