Development of a ³¹P magnetic resonance spectroscopy technique to quantify NADH and NAD⁺ at 3 T.

ABSTRACT: NADH and NAD⁺ act as electron donors and acceptors and NAD⁺ was shown to stimulate mitochondrial biogenesis and metabolic health. We here develop a non-invasive Phosphorous Magnetic Resonance Spectroscopy (³¹P-MRS) method to quantify these metabolites in human skeletal muscle on a clinical 3 T MRI scanner. This new MR-sequence enables NADH and NAD+ quantification by suppressing α-ATP signal, normally overlapping with NADH and NAD⁺. The sequence is based on a double spin echo in combination with a modified z-Filter achieving strong α-ATP suppression with little effect on NAD⁺ and NADH. Here we test and validate it in phantoms and in humans by measuring reproducibility and detecting a physiological decrease in NAD⁺ and increas

SUBMITTER: Mevenkamp J 

PROVIDER: S-EPMC11499639 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature