Rapid in situ carbon-13 hyperpolarization and imaging of acetate and pyruvate esters without external polarizer.

ABSTRACT: Hyperpolarized ¹³C MRI visualizes real-time metabolic processes in vivo. In this study, we achieved high ¹³C polarization in situ in the bore of an MRI system for precursor molecules of most widely employed hyperpolarized agents: [1-¹³C]acetate and [1-¹³C]pyruvate ethyl esters in their perdeuterated forms, enhancing hyperpolarization lifetimes, hyperpolarized to P_13C ≈ 28% at 80 mM concentration and P_13C ≈ 19% at 10 mM concentration, respectively. Using vinyl esters as unsaturated Parahydrogen-Induced Polarization via Side-Arm Hydrogenation (PHIP-SAH) precursors and our novel polarization setup, we achieved these hyperpolarization levels by fast side-arm hydrogenation in acetone-d₆ at elevated temperatures (up to

SUBMITTER: Mohiuddin O 

PROVIDER: S-EPMC11499913 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature