A cancer immunoprofiling strategy using mass spectrometry coupled with bioorthogonal cleavage.

ABSTRACT: The accurate quantification of biomarkers is paramount in modern medicine, particularly in cancer where precise diagnosis is imperative for targeted therapy selection. In this paper we described a multiplexed analysis diagnostic approach based on cleavable MS-tagged antibodies. The technology uses MS-tag isotopologues and the sydnonimine-cyclooctyne click-and-release bioorthogonal reaction. In a proof of concept study, we demonstrated the potential of this approach for cancer cell immunoprofiling in culture cells, tissues and in vivo as well, thereby unveiling promising diagnostic avenues.

SUBMITTER: Riberaud M 

PROVIDER: S-EPMC11499955 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature