Selective and naked eye colorimetric detection of creatinine through aptamer-based target-induced passivation of gold nanoparticles.

ABSTRACT: We report a simple naked eye colorimetric detection assay developed for the small molecule creatinine using the surface passivation of gold nanoparticles (AuNPs) which is conjugated with a creatinine binding aptamer. The selective binding of creatinine to aptamer sequences causes a decrease in the catalytic activity of AuNPs, and the color change time of the 4-nitrophenol reduction was used for the quantitative colorimetric detection of creatinine. Herein, the surfaces of AuNPs acted as the catalyst for the reduction of 4-nitrophenol (yellow) to 4-aminophenol (colorless), and the passivation with creatinine bound aptamer sequences delayed the reduction. The developed assay was able to detect creatinine in a linear range of 2-20 mM with a limit of detection of 0.87 mM. The developed colorim

SUBMITTER: Das C 

PROVIDER: S-EPMC11500065 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature