Assessing RNA integrity by digital RT-PCR: Influence of extraction, storage, and matrices.

ABSTRACT: The development of high-throughput sequencing has greatly improved our knowledge of microbial diversity in aquatic environments and its evolution in highly diverse ecosystems. Relevant microbial diversity description based on high-throughput sequencing relies on the good quality of the nucleic acid recovered. Indeed, long genetic fragments are more informative for identifying mutation combinations that characterize variants or species in complex samples. This study describes a new analytical method based on digital Polymerase Chain Reaction (PCR) partitioning technology for assessing the fragmentation of nucleic acid and more specifically viral RNA. This method allows us to overcome limits associated with hydrolysis probe-based assay by focusing on the distance between different amplicons,

SUBMITTER: Wurtzer S 

PROVIDER: S-EPMC11500190 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature