Development of inducible promoter and CRISPRi plasmids functional in <i>Rickettsia rickettsii</i>.

ABSTRACT: Rickettsia rickettsii is an obligate intracellular, tick-borne bacterium that causes Rocky Mountain spotted fever. The demanding nature of cultivating these bacteria within host cells and the labor involved in obtaining clonal isolates have severely limited progress regarding the development of compatible genetic tools to study this pathogen. Conditional expression of genes that might be toxic or have an otherwise undesirable effect is the next logical goal to expand upon the constitutive expression plasmids generated thus far. We describe the construction of an inducible promoter system based on the tet-On system, leveraging design elements from the anhydrotetracycline-inducible promoter system used for Borrelia burgdorferi and one of the few characterized rickettsial promot

SUBMITTER: Nock AM 

PROVIDER: S-EPMC11500500 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature