MRNA localization and thylakoid protein biogenesis in the filamentous heterocyst-forming cyanobacterium <i>Anabaena</i> sp. PCC 7120.

ABSTRACT: Heterocyst-forming cyanobacteria such as Anabaena (Nostoc) sp. PCC 7120 exhibit extensive remodeling of their thylakoid membranes during heterocyst differentiation. Here we investigate the sites of translation of thylakoid membrane proteins in Anabaena vegetative cells and developing heterocysts, using mRNA fluorescent in situ hybridization (FISH) to detect the location of specific mRNA species. We probed mRNAs encoding reaction center core components and the heterocyst-specific terminal oxidases Cox2 and Cox3. As in unicellular cyanobacteria, the mRNAs encoding membrane-integral thylakoid proteins are concentrated in patches at the inner face of the thylakoid membrane system, adjacent to the central cytoplasm. These patches mark the putative sites of translatio

SUBMITTER: Wang K 

PROVIDER: S-EPMC11500504 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature