Overexpression of diglucosyldiacylglycerol synthase leads to daptomycin resistance in <i>Bacillus subtilis</i>.

ABSTRACT: The lipopeptide antibiotic daptomycin exhibits bactericidal activity against Gram-positive bacteria by forming a complex with phosphatidylglycerol (PG) and lipid II in the cell membrane, causing membrane perforation. With the emergence of daptomycin-resistant bacteria, understanding the mechanisms of bacterial resistance to daptomycin has gained great importance. In this study, we aimed to identify the genetic factors contributing to daptomycin resistance in Bacillus subtilis, a model Gram-positive bacterium. Our findings demonstrated that overexpression of ugtP, which encodes diglucosyldiacylglycerol synthase, induces daptomycin resistance in B. subtilis. Specifically, overexpression of ugtP resulted in increased levels of diglucosyldiacylglycerol (Glc₂

SUBMITTER: Yamamoto R 

PROVIDER: S-EPMC11500525 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature