Inference and design of antibody specificity: From experiments to models and back.

ABSTRACT: Exquisite binding specificity is essential for many protein functions but is difficult to engineer. Many biotechnological or biomedical applications require the discrimination of very similar ligands, which poses the challenge of designing protein sequences with highly specific binding profiles. Experimental methods for generating specific binders rely on in vitro selection, which is limited in terms of library size and control over specificity profiles. Additional control was recently demonstrated through high-throughput sequencing and downstream computational analysis. Here we follow such an approach to demonstrate the design of specific antibodies beyond those probed experimentally. We do so in a context where very similar epitopes need to be discriminated, and where these epitopes cann

SUBMITTER: Fernandez-de-Cossio-Diaz J 

PROVIDER: S-EPMC11501025 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature