Improved localization precision via restricting confined biomolecule stochastic motion in single-molecule localization microscopy.

ABSTRACT: Single-molecule localization microscopy (SMLM) plays an irreplaceable role in biological studies, in which nanometer-sized biomolecules are hardly to be resolved due to diffraction limit unless being stochastically activated and accurately located by SMLM. For biological samples preimmobilized for SMLM, most biomolecules are cross-linked and constrained at their immobilizing sites but still expected to undergo confined stochastic motion in regard to their nanometer sizes. However, few lines of direct evidence have been reported about the detectability and influence of confined biomolecule stochastic motion on localization precision in SMLM. Here, we access the potential stochastic motion for each immobilized single biomolecule by calculating the displacements between any two of its localiz

SUBMITTER: Ni J 

PROVIDER: S-EPMC11501310 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature