Annular pupil confocal Brillouin-Raman microscopy for high spectral resolution multi-information mapping.

ABSTRACT: Brillouin-Raman combined confocal spectroscopy is a novel and powerful technique for providing non-contact and direct readout of the micro-regional chemical and mechanical properties of a material, and thus used in a broad range of applications, including material characterization in manufacturing and biological imaging. However, the inadequate spectral and spatial resolution restricts the further development of combined spectral technology. In this paper, an annular pupil confocal Brillouin-Raman microscopy (APCBRM) scheme is proposed to achieve high-spectral-resolution Brillouin spectral detection and high-lateral-resolution Brillouin, Raman, and 3D topography mapping. The use of an annular pupil significantly suppresses the spectral broadening caused by a high-numerical-aperture objecti

SUBMITTER: Su Y 

PROVIDER: S-EPMC11501813 | biostudies-literature | 2023 Sep

REPOSITORIES: biostudies-literature