Super-resolved three-dimensional near-field mapping by defocused imaging and tracking of fluorescent emitters.

ABSTRACT: Near-field optics is essential in many nanotechnology applications, such as implementing sensitive biosensing and imaging systems with extreme precision. Understanding optical near-fields at the nanoscale has so attracted the considerable research interest, which use a variety of analytical approaches, most notably near-field scanning microscopy. Here, we show defocused point localization mapped accumulation (DePLOMA), which can overcome many weaknesses of conventional analytical methods. DePLOMA is based on imaging fluorescence emitters at an out-of-focal plane. The acquisition, collection, and accumulation of the position and fluorescence intensity of emitters moving above nanostructures can generate three-dimensional near-field maps of light distribution. The idea enables super-resoluti

SUBMITTER: Son T 

PROVIDER: S-EPMC11501887 | biostudies-literature | 2022 Dec

REPOSITORIES: biostudies-literature