CAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis.

ABSTRACT: Promoters, including neither TATA box nor initiator, have been frequently found in testicular germ cell-specific genes in mice. These investigations imply that unique forms of the polymerase II transcription initiation machinery play a role in selective activation of germ cell-specific gene expression programs during spermatogenesis. However, there is little information about testis-specific core promoters, because useful germ cell culture system is not available. In this study, we characterize the regulatory region of the haploid-specific Oxct2b gene in detail by using in vivo transient transfection assay in combination with a transgenic approach, with electrophoretic mobility shift and chromatin immunoprecipitation assays. Expression studies using mutant constructs demonstrate that a 34

SUBMITTER: Somboonthum P 

PROVIDER: S-EPMC1150221 | biostudies-literature | 2005

REPOSITORIES: biostudies-literature