Protein kinase D1 in myeloid lineage cells contributes to the accumulation of CXCR3⁺CCR6⁺ nonconventional Th1 cells in the lungs and potentiates hypersensitivity pneumonitis caused by <i>S. rectivirgula</i>.

ABSTRACT:

Introduction

Hypersensitivity pneumonitis (HP) is an extrinsic allergic alveolitis characterized by inflammation of the interstitium, bronchioles, and alveoli of the lung that leads to granuloma formation. We previously found that activation of protein kinase D1 (PKD1) in the lungs following exposures to Saccharopolyspora rectivirgula contributes to the acute pulmonary inflammation, IL-17A expression in the lungs, and development of HP. In the present study, we investigated whether PKD1 in myeloid-lineage cells affects the pathogenic course of the S. rectivirgula-induced HP.

Methods

Mice were exposed intranasally to S. rectivirgula once or 3 times/week for 3 weeks. The protein and mRNA expression levels of cytokines/chemokines were detected by enzyme-lin

SUBMITTER: Snyder JD 

PROVIDER: S-EPMC11502317 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature