Triplex real-time qPCR for the simultaneous detection of <i>Botryosphaeriaceae</i> species in woody crops and environmental samples.

ABSTRACT:

Introduction

Species of Botryosphaeriaceae fungi are relevant pathogens of almond causing trunk cankers, extensive gumming, necrosis of internal tissues and plant dieback and dead, threatening almond productivity. A novel triplex quantitative real-time PCR (qPCR) assay was designed for the simultaneous detection and quantification of Neofusicoccum parvum, Botryosphaeria dothidea and the Botryosphaeriaceae family.

Material and methods

The method was validated in symptomatic and asymptomatic almond, avocado, blueberry and grapevine plants and in environmental samples, such as cropping soil and rainwater and in artificially inoculated trapped spores, demonstrating the same performance on several matrices.

Results and discussion

The limit of de

SUBMITTER: Romero-Cuadrado L 

PROVIDER: S-EPMC11502354 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature