Lithium rescues cultured rat metatarsals from dexamethasone-induced growth failure.

ABSTRACT:

Background

Glucocorticoids are commonly used in children with different chronic diseases. Growth failure represents a so far untreatable undesired side-effect. As lithium chloride (LiCl) is known to induce cell renewal in various tissues, we hypothesized that LiCl may prevent glucocorticoid-induced growth failure.

Methods

We monitored growth of fetal rat metatarsals cultured ex-vivo with dexamethasone and/or LiCl, while molecular mechanisms were explored through RNA sequencing by implementing the differential gene expression and gene set analysis. Quantification of β-catenin in human growth plate cartilage cultured with dexamethasone and/or LiCl was added for verification.

Results

After 14 days of culture, the length of dexamethasone-treated fetal rat metatarsals incr

SUBMITTER: Soucek O 

PROVIDER: S-EPMC11502490 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature