Characterization of the phagocytic ability of white blood cells separated using a single curvature spiral microfluidic device.

ABSTRACT: The present work describes a microfluidic device developed for separating white blood cells (WBCs) for the Nitroblue Tetrazolium (NBT) bioassay, which quantifies the phagocytic ability of cells. The NBT test requires a small number of phagocytic cells but is highly susceptible to the presence of red blood cells (RBCs). Our inertial microfluidic device can deliver a WBC sample by removing 99.99% of RBCs and subsequently reducing the ratio of RBC to WBC from 848:1 to 2:3. The microdevice operates on a relatively higher hematocrit concentration (1% Hct) of blood. Compared to conventional WBC separation methods, the microdevice's passive, label-free nature preserves the cell properties of the original sample. A single-turn spiral microfluidic device with a rectangular cross-section is simple t

SUBMITTER: Mane S 

PROVIDER: S-EPMC11502713 | biostudies-literature | 2024 Nov

REPOSITORIES: biostudies-literature