Hydrogen isotope labeling unravels origin of soil-bound organic contaminant residues in biodegradability testing.

ABSTRACT: Biodegradability testing in soil helps to identify safe synthetic organic chemicals but is still obscured by the formation of soil-bound 'non-extractable' residues (NERs). Present-day methodologies using radiocarbon or stable (¹³C, ¹⁵N) isotope labeling cannot easily differentiate soil-bound parent chemicals or transformation products (xenoNERs) from harmless soil-bound biomolecules of microbial degraders (bioNERs). Hypothesizing a minimal retention of hydrogen in biomolecules, we here apply stable hydrogen isotope - deuterium (D) - labeling to unravel the origin of NERs. Soil biodegradation tests with D- and ¹³C-labeled 2,4-D, glyphosate and sulfamethoxazole reveal consistently lower proportions of applied D than ¹³C in total NERs and in amino a

SUBMITTER: Lennartz S 

PROVIDER: S-EPMC11502848 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature