DNA methylation stability in cardiac tissues kept at different temperatures and time intervals.

ABSTRACT: Investigating DNA methylation (DNAm) in cardiac tissues is vital for epigenetic research in cardiovascular diseases (CVDs). During cardiac surgery, biopsies may not be immediately stored due to a lack of human or technical resources at the collection site. Assessing DNAm stability in cardiac samples left in suboptimal conditions is crucial for applying DNAm analysis. We investigated the stability of DNAm in human cardiac tissues kept at 4 °C and 22 °C for periods of 1, 7, 14, and 28 days (exposed samples) using the Illumina Infinium MethylationEPIC v1.0 BeadChip Array. We observed high correlations between samples analysed immediately after tissue collection and exposed ones (R² > 0.992). Methylation levels were measured as β-values and median absolute β-value differences (|∆β|)

SUBMITTER: Poggiali B 

PROVIDER: S-EPMC11502879 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature