Itaconate is a metabolic regulator of bone formation in homeostasis and arthritis.

ABSTRACT:

Objectives

Bone remodelling is a highly dynamic process dependent on the precise coordination of osteoblasts and haematopoietic-cell derived osteoclasts. Changes in core metabolic pathways during osteoclastogenesis, however, are largely unexplored and it is unknown whether and how these processes are involved in bone homeostasis.

Methods

We metabolically and transcriptionally profiled cells during osteoclast and osteoblast generation. Individual gene expression was characterised by quantitative PCR and western blot. Osteoblast function was assessed by Alizarin red staining. immunoresponsive gene 1 (Irg1)-deficient mice were used in various inflammatory or non-inflammatory models of bone loss. Tissue gene expression was analysed by RNA in situ hybridisation.

SUBMITTER: Kieler M 

PROVIDER: S-EPMC11503170 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature