RNA m⁶A modification regulates cell fate transition between pluripotent stem cells and 2-cell-like cells.

ABSTRACT: N⁶-methyladenosine (m⁶A) exerts essential roles in early embryos, especially in the maternal-to-zygotic transition stage. However, the landscape and roles of RNA m⁶A modification during the transition between pluripotent stem cells and 2-cell-like (2C-like) cells remain elusive. Here, we utilised ultralow-input RNA m⁶A immunoprecipitation to depict the dynamic picture of transcriptome-wide m⁶A modifications during 2C-like transitions. We found that RNA m⁶A modification was preferentially enriched in zygotic genome activation (ZGA) transcripts and MERVL with high expression levels in 2C-like cells. During the exit of the 2C-like state, m⁶A facilitated the silencing of ZGA genes and MERVL. Notably, inhibition of m

SUBMITTER: Su Z 

PROVIDER: S-EPMC11503247 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature