Association Between Dairy Production System and Milk Functionality Based on Analysis of miRNAs in Exosomes from Milk.

ABSTRACT: Dairy farming practices significantly affect the nutritional and functional properties of milk. This study compares miRNAs in milk exosomes from extensive and intensive dairy systems and explores their potential implications for human consumers. Extensive systems are believed to produce milk of higher quality with better animal welfare compared to intensive systems. Milk samples from eight extensive and nine intensive dairy farms were analysed. Milk-derived extracellular vesicles were isolated through sequential ultracentrifugation and characterised through Dynamic Light Scattering and Nanosight to determine the size and the concentration of the extracellular vesicles, in addition to immunoblotting to ensure the presence of exosome-specific proteins in their membrane. miRNA levels were qua

SUBMITTER: Abou El Qassim L 

PROVIDER: S-EPMC11503750 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature