Peptide-Based Turn-On Fluorescent Probes for Highly Specific Detection of Survivin Protein in the Cancer Cells.

ABSTRACT: Survivin is highly expressed in most human cancers, making it a promising target for cancer diagnosis and treatment. In this study, we developed peptide probes consisting of Bor_65-75, a high-affinity survivin-binding peptide, and a survivin protein segment using peptide linkers as survivin-sensitive fluorescent probes (SSFPs). All conjugates were attached to 5(6)-carboxyfluorescein (FAM) at the C-terminal as a fluorophore and to 4((4(dimethylamino)phenyl)azo)benzoic acid (DABCYL) at the N-terminal as a quencher. Fluorescence (or Förster) resonance energy transfer (FRET) quenching via intramolecular binding of Bor_65-75 with survivin protein segment could be diminished by the approach of survivin to SSFPs, which dissociate Bor_65-75 from SSPF and

SUBMITTER: Fuchigami T 

PROVIDER: S-EPMC11504145 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature