3D Optical Reconstruction of the Nervous System of the Whole-Body Marine Invertebrates.

ABSTRACT: Optical clearing of invertebrates, the number of species of which is 20 times greater than that of vertebrates, is of fundamental and applied interest for neuroscience in general. Herein, the optical clearing of invertebrates to identify their morphology and neurostructure remains unrealized as of yet. Here, we report on fast (from a few seconds to minutes) and uniform whole-body optical clearing of invertebrates (bivalves, nemertines, annelids, and anomura) of any age and thickness (up to 2 cm) possessing complicated structures and integuments. We developed the protocol unifying dimethyl sulfoxide (DMSO)-based immunostaining of the animals followed by their optical clearing with benzyl alcohol/benzyl benzoate (BABB). Confocal microspectroscopy revealed that the protocol provides an increa

SUBMITTER: Milichko VA 

PROVIDER: S-EPMC11504524 | biostudies-literature | 2023 Dec

REPOSITORIES: biostudies-literature