Genomic Characterization of 16S rRNA Methyltransferase-Producing <i>Enterobacterales</i> Reveals the Emergence of <i>Klebsiella pneumoniae</i> ST6260 Harboring <i>rmtF</i>, <i>rmtB</i>, <i>bla</i>_NDM-5, <i>bla</i>_OXA-232 and <i>bla</i>_SFO-1 Genes in a Cancer Hospital in Bulgaria.

ABSTRACT: Background: Acquired 16S rRNA methyltransferases (16S-RMTases) confer high-level resistance to aminoglycosides and are often associated with β-lactam and quinolone resistance determinants. Methods: Using PCR, whole-genome sequencing and conjugation experiments, we conducted a retrospective genomic surveillance study of 16S-RMTase-producing Enterobacterales, collected between 2006 and 2023, to explore transmission dynamics of methyltransferase and associated antibiotic resistance genes. Results: Among the 10,731 consecutive isolates, 150 (1.4%) from 13 species carried armA (92.7%), rmtB (4.7%), and rmtF + rmtB (2.7%) methyltransferase genes. The coexistence of extended-spectrum β-lactamase (bla_CTX-M-3/15, bla

SUBMITTER: Sabtcheva S 

PROVIDER: S-EPMC11504970 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature