Flow Cytometric Immunophenotyping: Minimal Differences in Fresh and Cryopreserved Peripheral Blood Mononuclear Cells versus Whole Blood.

ABSTRACT: Background/Objectives: Flow cytometry is a convenient tool in immunophenotyping for monitoring the status of immunological conditions and diseases. The aim of this study was to investigate the effect of isolation and cryopreservation by flow cytometric analysis on subpopulations of CD4⁺ T helper (Th), T regulatory (Treg), CD8⁺ T cytotoxic (Tc), CD56⁺ NK, CD19⁺ B and monocytes. Freshly isolated and cryopreserved peripheral blood mononuclear cells (PBMCs) were compared to fresh whole blood. Methods: Peripheral blood was collected from healthy donors and prepared for flow cytometric analysis using the same panels of antibodies throughout the study. Results: Comparisons between fresh (F)- and cryopreserved (C)-PBMCs showed no maj

SUBMITTER: Tompa A 

PROVIDER: S-EPMC11505181 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature