Development of a High-Resolution Melting Method for the Detection of Clarithromycin-Resistant <i>Helicobacter pylori</i> in the Gastric Microbiome.

ABSTRACT: Background: The issue of Helicobacter pylori (H. pylori) resistance to clarithromycin (CLR) has consistently posed challenges for clinical treatment. Hence, a rapid susceptibility testing (AST) method urgently needs to be developed. Methods: In the present study, 35 isolates of H. pylori were isolated from 203 gastritis patients of the Guangzhou cohort, and the antimicrobial resistance phenotypes were associated with their genomes to analyze the relevant mutations. Based on these mutations, a rapid detection system utilizing high-resolution melting (HRM) curve analysis was designed and verified by the Shenzhen cohort, which consisted of 38 H. pylori strains. Results: Genomic analysis identified the mutation of the 2143 allele from A to G (A2

SUBMITTER: Kuang Z 

PROVIDER: S-EPMC11505316 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature