Distinguishing Protein Corona from Nanoparticle Aggregate Formation in Complex Biological Media Using X-ray Photon Correlation Spectroscopy.

ABSTRACT: In biological systems, nanoparticles interact with biomolecules, which may undergo protein corona formation that can result in noncontrolled aggregation. Therefore, comprehending the behavior and evolution of nanoparticles in the presence of biological fluids is paramount in nanomedicine. However, traditional lab-based colloid methods characterize diluted suspensions in low-complexity media, which hinders in-depth studies in complex biological environments. Here, we apply X-ray photon correlation spectroscopy (XPCS) to investigate silica nanoparticles (SiO₂) in various environments, ranging from low to high complex biological media. Interestingly, SiO₂ revealed Brownian motion behavior, irrespective of the complexity of the chosen media. Moreover, the SiO₂

SUBMITTER: Silva CEP 

PROVIDER: S-EPMC11505373 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature