The Internalization Pathways of Liposomes, PLGA, and Magnetic Nanoparticles in Neutrophils.

ABSTRACT:

Background/objectives

Neutrophils are emerging as promising candidates for cell-based nanodrug delivery to tumors due to their unique biological properties. This study aims to investigate the mechanisms of nanoparticle internalization by neutrophils, specifically focusing on liposomes, poly(lactic-co-glycolic acid) (PLGA), and magnetite nanoparticles. Understanding these mechanisms could enhance the efficiency of neutrophil-based nanodrug delivery for cancer treatment.

Methods

Neutrophils were isolated from the peripheral blood of mice bearing 4T1 mammary adenocarcinoma. Confocal microscopy, transmission electron microscopy, and flow cytometry were employed to evaluate the uptake of liposomes, PLGA, and magnetite nanoparticles by neutrophils. The effects of cultivation condi

SUBMITTER: Garanina A 

PROVIDER: S-EPMC11505478 | biostudies-literature | 2024 Sep

REPOSITORIES: biostudies-literature