Heterogeneous Nuclear Ribonucleoprotein A1 Knockdown Alters Constituents of Nucleocytoplasmic Transport.

ABSTRACT:

Background/objectives

Changes in nuclear morphology, alterations to the nuclear pore complex (NPC), including loss, aggregation, and dysfunction of nucleoporins (Nups), and nucleocytoplasmic transport (NCT) abnormalities have become hallmarks of neurodegenerative diseases. Previous RNA sequencing data utilizing knockdown of heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) identified enrichment for pathways and changes in RNAs related to nuclear morphology and showed differential expression of key nuclear targets. This suggests that dysfunction of hnRNP A1, which is observed in neurodegenerative diseases, may contribute to abnormalities in nuclear morphology, NPC, and NCT.

Methods

We performed knockdown of hnRNP A1 in Neuro-2A cells, a neuronal cell line, to examine nucl

SUBMITTER: Stang TE 

PROVIDER: S-EPMC11505608 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature