Impact of Nitric Oxide on Polymorphonuclear Neutrophils' Function.

ABSTRACT:

Background

There is increasing evidence that nitric oxide (nitrogen monoxide, NO) significantly influences immune cellular responses, including those from polymorphonuclear leukocytes (PMNs).

Objective

The aim of this study was to examine a possible effect of NO on PMNs' function (chemotaxis, production of reactive oxygen species (ROS), and NETosis) using live cell imaging. Moreover, we investigated PMN surface epitope and neutrophil oxidative burst under the influence of NO by flow cytometric analysis.

Methods

Whole blood samples were obtained from healthy volunteers, and PMNs were isolated by density centrifugation. Live cell imaging using type I collagen matrix in µSlide IBIDI chemotaxis chambers was conducted in order to observe N-formyl-L-methionyl-L-leucyl-pheny

SUBMITTER: Kraus R 

PROVIDER: S-EPMC11505631 | biostudies-literature | 2024 Oct

REPOSITORIES: biostudies-literature