Indigo alleviates psoriasis through the AhR/NF-κB signaling pathway: an <i>in vitro</i> and <i>in vivo</i> study.

ABSTRACT:

Background

Psoriasis is a chronic inflammatory skin disease. A strong association between the AhR/ NFκB axis and the inflammatory response in psoriasis. Indigo (IDG) has demonstrated significant anti-inflammatory properties. This study aimed to assess the anti-psoriatic efficacy of IDG while investigating the underlying mechanisms involved.

Methods

In the in vitro experiments, cell viability was assessed using the CCK-8. qRT-PCR was employed to measure the mRNA levels of NF-κB, TNF-α, IL-1β, AhR, and CYP1A1. Western blotting was conducted to examine alterations in cytoplasmic and nuclear AhR protein levels. Additionally, an IDG nanoemulsion (NE) cream was prepared for the in vivo experiments. A psoriasis-like skin lesion mice model was induced using IMQ (62.5 m

SUBMITTER: Lin Y 

PROVIDER: S-EPMC11505883 | biostudies-literature | 2024

REPOSITORIES: biostudies-literature